Aptagen, LLC
RNA
Pepocin
Protein
18 nM (reported value)
Binding buffer: 3 mM NaH2PO4, 3 mM MgCl2, 45 mM NaCl, 2.5 mM EDTA, 3 mM Tris-HCl (pH 7.5), 0.15 mM DTT, and 12% glycerol.
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Pepocin (9-41U22) (ID# 171)
5'rGprGprGprAprGprUprCprUprGprAprAprGprUprCprGprGprAprCprUprUprGprUprUprAprUprCprAprAprUprUprCprAprCprUprUprCprAprGprAprCprUp3'
41
13103.89 g/mole
410700 L/(mole·cm)
43.90%
2.43
31.91
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Hirao et al. "RNA Aptamers That Bind to and Inhibit the Ribosome-inactivating Protein, Pepocin." The Journal of Biological Chemistry, 275(2000): 4943-4948.
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