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Apta-Index™: C-Reactive Protein Monomer (ID# 164)

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C-Reactive Protein Monomer (ID# 164)

C-Reactive Protein Monomer
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Monomeric C-Reactive Protein (CRP) Protein 187.7 nM (reported value) Binding Buffer: 20 mM HEPES, 140 mM NaCl, 50 mM KCl, 0.1 mM DTT, and 5% glycerol at pH 6.5
Incubated for 20 min.
N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. M S Wang (2012) confirmed monomeric binding using a gel shift assay.
44 14346.65 g/mole 444700.00 L/(mole·cm) 56.82% 2.25 32.26

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

M S Wang et al. "C-reactive protein (CRP) aptamer binds to monomeric but not pentameric form of CRP." Analytical and Bioanalytical Chemistry, 401(2011): 1309-1318.

M S Wang and S M Reed. Direct visualization of electrophoretic mobility shift assays using nanoparticle-aptamer conjugates. Electrophoresis. 33(2012): 348-351.

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