Aptagen, LLC
RNA
Monomeric C-Reactive Protein (CRP)
Protein
187.7 nM (reported value)
Binding Buffer: 20 mM HEPES, 140 mM NaCl, 50 mM KCl, 0.1 mM DTT, and 5% glycerol at pH 6.5 Incubated for 20 min.
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Selectively binds to monomeric CRP and not pentameric CRP.
M S Wang (2012) confirmed monomeric binding using a gel shift assay.
C-Reactive Protein Monomer (ID# 164)
5'rGprCprCprUprGprUprAprAprGprGprUprGprGprUprCprGprGprUprGprUprGprGprCprGprAprGprUprGprUprGprUprUprAprGprGprAprGprAprGprAprUprUprGprCp3'
44
14346.65 g/mole
444700 L/(mole·cm)
56.82%
2.25
32.26
M S Wang et al. "C-reactive protein (CRP) aptamer binds to monomeric but not pentameric form of CRP." Analytical and Bioanalytical Chemistry, 401(2011): 1309-1318.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
M S Wang et al. "C-reactive protein (CRP) aptamer binds to monomeric but not pentameric form of CRP." Analytical and Bioanalytical Chemistry, 401(2011): 1309-1318.
M S Wang and S M Reed. Direct visualization of electrophoretic mobility shift assays using nanoparticle-aptamer conjugates. Electrophoresis. 33(2012): 348-351.
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