* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA 6X His Tag Protein 4.6 nM (reported value)Selection Buffer (50mM K2HPO4, 150mM NaCl, 0.05% Tween 20 (pH 7.5))N/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kokpinar, et al. "Aptamer-Based Downstream Processing of His-Tagged Proteins Utilizing Magnetic Beads." Biotechnology and Bioengineering, 108(2011):2371-2379.
Have your aptamer oligo synthesized by one of our AFFILIATES
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
DNA
6X His Tag
Protein
4.6 nM (reported value)
Selection Buffer (50mM K2HPO4, 150mM NaCl, 0.05% Tween 20 (pH 7.5))
N/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
