Aptagen, LLC
DNA
6X His Tag
Protein
4.6 nM (reported value)
Selection Buffer (50mM K2HPO4, 150mM NaCl, 0.05% Tween 20 (pH 7.5))
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Anti-His Tag (6H7) (ID# 163)
5'dGpdCpdTpdApdTpdGpdGpdGpdTpdGpdGpdTpdCpdTpdGpdGpdTpdTpdGpdGpdGpdApdTpdTpdGpdGpdCpdCpdCpdCpdGpdGpdGpdApdGpdCpdTpdGpdGpdCp3'
40
12488.1 g/mole
370000 L/(mole·cm)
67.50%
2.70
33.75
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Kokpinar, et al. "Aptamer-Based Downstream Processing of His-Tagged Proteins Utilizing Magnetic Beads." Biotechnology and Bioengineering, 108(2011):2371-2379.
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