Aptagen, LLC
RNA
Human Activated Protein C
Protein
137 nM (reported value)
Binding Buffer: 10 mM sodium citrate, 150 mM KCl (pH 7.6).
Incubation Time: 10 min.
Refolding Protocol: Heated at 70°C for 3 min then cooled at RT for 10 min.
Assay Type: Nitrocellulose filter. RT°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Strong serine protease inhibitor of APC.
Activated Protein C (APC 99) (ID# 134)
Incubation Time: 10 min.
Refolding Protocol: Heated at 70°C for 3 min then cooled at RT for 10 min.
Assay Type: Nitrocellulose filter. RT°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Strong serine protease inhibitor of APC.
5'rGprUprGp rAprGprAp rCprCprAp rGprCprCp rGprAprGp rUprGprGp rUprGprUp rCprUprGp rGprCprUp rAprUprUp rCprAprCp rUprGprGp rAprGprCp rGprUprGp rGprGprUp rGprGprAp rAprCprCp rCprCprUp rGprCprGp rCprAprCp rUprCprGp rUprUprUp rGprGprCp rUprGprUp rCprCprGp rGprGprCp rCprUprUp rCprGprGp rGprCprCp rGprGprGp rAprUprUp rAprUprCp rUprCprUp3'
110
31865.02 g/mole
931000 L/(mole·cm)
62.63%
1.07
34.23
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Gal, S., et al. "Selection of a RNA aptamer that binds to human activated protein C and inhibits its protease function." European Journal of Biochemistry, 252(1998): 553-562.
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