* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Human Activated Protein C Protein 137 nM (reported value)Binding Buffer: 10 mM sodium citrate, 150 mM KCl (pH 7.6).
Incubation Time: 10 min.
Refolding Protocol: Heated at 70°C for 3 min then cooled at RT for 10 min.
Assay Type: Nitrocellulose filter.N/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.Strong serine protease inhibitor of APC.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Gal, S., et al. "Selection of a RNA aptamer that binds to human activated protein C and inhibits its protease function." European Journal of Biochemistry, 252(1998): 553-562.
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* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA
Human Activated Protein C
Protein
137 nM (reported value)
Binding Buffer: 10 mM sodium citrate, 150 mM KCl (pH 7.6).
