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Apta-Index™: HIV-1 Nuceocapsid (ID# 132)

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HIV-1 Nuceocapsid (ID# 132)

HIV-1 Nuceocapsid
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA HIV-1 Nuceocapsid Protein 2.3 nM (reported value) Binding Buffer (50 mM Tris, pH 7.5, 100 mM NaCl, 1 mM MgCl2, 30 µM ZnCl and 10 mM DTT) SSC buffer (17.5 mM sodium citrate (pH 7.0), 150 mM sodium chloride) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprAprGprAprCprAprUprCprCprCprUprCprUprUprGprUprUprGprGprUprGprGprUprGprCprCprGprUprGprUprGprGprAprUprGprUprCprUprCp-3'
40 12814.65 g/mole 381200.00 L/(mole·cm) 57.50% 2.62 33.62

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Berglund, A., et al. "A High Affinity Binding Site for the HIV-1 Nucleocapsid Protein." Nucleic Acids Research, 25(1997):1042-1049.

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