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Apta-Index™: Hepatitis C NS3 Protein (10G-1) (ID# 130)

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Hepatitis C NS3 Protein (10G-1) (ID# 130)

Hepatitis C NS3 Protein (10G-1)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Hepatitis C NS3 Protein Protein 990 pM (reported value) Binding Buffer: 50 mM Tris/HCl (pH 7.7), 30 mM NaCl, 5 mM CaCl2, 10 mM dithiothreitol and 1% BSA Elution Buffer (200 uL of 0.4 M sodium acetate, 5 mM EDTA, and 7 M urea (pH 5.5)) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. An assay of the inhibition of helicase activity by the aptamer showed an IC50 of 12.5 nM.
5'-rGprGprGprAprGprAprGprCprGprGprAprAprGprCprGprUprGprCprUprGprGprGprCprCp rAprGprUprAprGprUprGprUprAprUprAprGprGprGprCprUprCprGprAprAprAprUprGprUprUprCprAprUprGprGprCprUprCprAprGprUprGprGprAp rCprAprUp -3'
66 21495.01 g/mole 665000.00 L/(mole·cm) 56.06% 1.50 32.32

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Urvil, P., et al. "Selection of RNA aptamers that bind specifically to the NS3 protease of hepatitis C virus." European Journal of Biochemistry, 248(1997):130-138.

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