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Apta-Index™: HIV-1 Integrase (P5) (ID# 129)

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HIV-1 Integrase (P5) (ID# 129)

HIV-1 Integrase (P5)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA HIV-1 Integrase Protein 10 nM (reported value) Buffer A (50 mM HEPES, 1 mM DTT, 1 mM APMSF) Binding Buffer (50 mM Na-HEPES (pH 7.5), 250 mM NaCl, 2 mM DTT, 10 mM MnCl2, 5 mM CHAPS) N/A If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
5'-rGprGprGprAprGprCprUprCprAprGprAprAprUprAprAprAprCprGprCprUprCprAprAprCprCprAprGprUprCprUprUprGprUprGprGprCprUprUprUprGprAprAprAprGprAprGprAprGprGprAprGprUprGprUprUprCprGprAprCprAprUprGprAprGprGprCprCprCprGprGprAprUprCprCprGprGprCp-3'
77 24933.10 g/mole 767300.00 L/(mole·cm) 54.55% 1.30 32.49

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Allen, P., et al. "Isolation of High-Affinity RNA Ligands to HIV-1 Integrase from a Random Pool." Virology, 209(1995): 327-336.

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