Human Influenza A virus H3N2 (P30-10-16) (ID# 121)
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA Human Influenza A virus H3N2 Cells .188 nM (reported value)Binding Buffer (20 nM HEPES/KOH (pH 7·4), 105 mM NaCl)
PIPES buffer (5 mM PIPES/NaOH, 145 mM NaCl (pH 7·5))
Acidic buffer (145 mM NaCl, 20 mM sodium citrate, 1 U RNase inhibitor µl-1 (pH 5·0))N/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Gopinath, S., et al. "An RNA aptamer that distinguishes between closely related human influenza viruses and inhibits haemagglutinin-mediated membrane fusion." Journal of General Virology, 87(2005): 479-487.
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.png "Human Influenza A virus H3N2 (P30-10-16)")
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
RNA
Human Influenza A virus H3N2
Cells
.188 nM (reported value)
Binding Buffer (20 nM HEPES/KOH (pH 7·4), 105 mM NaCl)
PIPES buffer (5 mM PIPES/NaOH, 145 mM NaCl (pH 7·5))
Acidic buffer (145 mM NaCl, 20 mM sodium citrate, 1 U RNase inhibitor µl-1 (pH 5·0))
N/A
If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
