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Tetracycline (cb28 minimer) (ID# 116)

Aptamer Chemistry: RNA Target: Tetracycline Antigen/Target Category: Small Organic Affinity (Kd): ~1 µM (reported value) Binding Conditions/Buffer: Equilibration Buffer: 10 mM Trisƒ??HCl (pH 7.6), 5 mM MgCl₂, 250 mM NaCl Binding Temp: RT°C Refolding Protocol: Heated to 90°C for 3 min and cooled at RT for 5 min. Specificity: Binds to tetracycline analogues 4-epi-tetracycline, oxytetracycline, and 7-chlortetracycline. Does not effectively bind to doxycycline or minocycline. Comments:

Sequence (): 5'rGprGprCprCprUprAprAprAprAprCprAprUprAprCprCprAprGprAprUprUprUprCprGprAprUprCprUprGprGprAprGprAprGprGprUprGprAprAprGprAprAprUprUprCprGprAprCprCprAprCprCprUprAprGprGprCprCprGprGprUp3' Length: 60 Molecular Weight: 19333.74 g/mole Extinction Coefficient: 604400 L/(mole·cm) GC Content: 50.00% nmoles/OD₂₆₀: 1.65 μg/OD₂₆₀: 31.99

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Berens C, Thain, A, Schroeder R. (2001) A tetracycline-binding RNA aptamer. Bioorg Med Chem. 9:2549-2556.

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Aptamer Details

Tetracycline (cb28 minimer) (ID# 116)

Berens C, Thain, A, Schroeder R. (2001) A tetracycline-binding RNA aptamer. Bioorg Med Chem. 9:2549-2556.

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