Aptamer Details

Aptamer MA (ID# 7233)

Aptamer Chemistry: DNA Target: methylenedioxypyrovalerone (MDPV) Antigen/Target Category: Small Organic Affinity (Kd): 7 nM (reported value) Binding Conditions/Buffer: 10 mM Tris–HCl, 0.5 mM MgCl2, 20 mM NaCl, pH 7.4 Binding Temp: 23°C Refolding Protocol: heated at 95◦C for 5min, slowly cooled to room temperature over 30 min Specificity: Highly Specific Comments: This aptamer intrinsically binds Cy7 within its TWJ domain, but MDPV efficiently displaces the dye, resulting in a change in absorbance within seconds. This allows this aptamer to act as a label free assay.

Sequence (): 5'dCpdTpdTpdApdCpdGpdApdCpdTpdCpdApdGpdGpdCpdApdTpdTpdTpdTpdGpdCpdCpdGpdGpdGpdTpdApdApdCpdGpdApdApdGpdTpdTpdApdCpdTpdGpdTpdCpdGpdTpdApdApdGp3' Length: 46 Molecular Weight: 14134.82 g/mole Extinction Coefficient: 441918 L/(mole·cm) GC Content: 47.83% nmoles/OD₂₆₀: 2.26 μg/OD₂₆₀: 31.99

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Haixiang Yu, Weijuan Yang, Obtin Alkhamis, Juan Canoura, Kyung-Ae Yang, Yi Xiao, In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality, Nucleic Acids Research, Volume 46, Issue 8, 4 May 2018, Page e43, https://doi.org/10.1093/nar/gky026

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Aptamer Details

Aptamer MA (ID# 7233)

Haixiang Yu, Weijuan Yang, Obtin Alkhamis, Juan Canoura, Kyung-Ae Yang, Yi Xiao, In vitro isolation of small-molecule-binding aptamers with intrinsic dye-displacement functionality, Nucleic Acids Research, Volume 46, Issue 8, 4 May 2018, Page e43, https://doi.org/10.1093/nar/gky026

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