Aptamer Details

#AP-Cell 1 (ID# 7203)

Aptamer Chemistry: DNA Target: Aspartate β-Hydroxylase (ASPH) Antigen/Target Category: Protein Affinity (Kd): 47.51 ± 5.86 nM (reported value) Binding Conditions/Buffer: 5 mM MgCl2, 4.5 g of glucose, 1 g BSA, and 100 mg yeast tRNA in 1 L Dulbecco’s PBS Binding Temp: 23°C Refolding Protocol: 95C for 5 minutes, snap-cool on ice Specificity: No binding to untransfected control HeLa cells. Comments:

Sequence (): 5'dCpdGpdGpdGpdApdCpdApdApdGpdApdCpdApdApdCpdGpdApdApdCpdGpdApdApdCpdApdGpdGpdApdApdGpdApdGpdApdApdCpdCpdGpdGpdApdApdTpdGpdCpdApdGpdApdCpdGpdTpdCpdApdGpdGpdGp3' Length: 52 Molecular Weight: 15879.27 g/mole Extinction Coefficient: 517300 L/(mole·cm) GC Content: 55.77% nmoles/OD₂₆₀: 1.93 μg/OD₂₆₀: 30.70

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Hadi Bakhtiari, Abbas Ali Palizban, Hossein Khanahmad, and Mohammad Reza Mofid ACS Omega 2021 6 (16), 11005-11014 DOI: 10.1021/acsomega.1c00876

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Aptamer Details

#AP-Cell 1 (ID# 7203)

Hadi Bakhtiari, Abbas Ali Palizban, Hossein Khanahmad, and Mohammad Reza Mofid ACS Omega 2021 6 (16), 11005-11014 DOI: 10.1021/acsomega.1c00876

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