Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA
PrP Fibrils
Protein
23.4 nM (reported value)
Binding Buffer (20 mM Hepes (pH 7.2), 100 mM NaCl, 50 mM KCl, 10 mM MgCl2)
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
The oligonucleotides used in this study were documented in this reference: Tahiri-Alaoui, A., et al. "High affinity nucleic acid aptamers for streptavidin incorporated into bi-specific capture ligands." Nucleic Acids Research, 30 (2002): e45
PrP Fibrils (SAF-93) (ID# 72)
5'rGprGprGprAprGprApfCprAprAprGprApfCpfUprAprGprApfCprGpfCpfUpfCprAprApfCpfUprApfCprGprAprApfCpfUpfCprApfUprGprApfCprApfCprAprAprGprGprApfUprGpfCprAprApfUpfCpfUpfCprApfUpfCpfCpfCprGpfCpfCprAprGpfCpfCpfCprApfCpfCprGpfUpfUpfUpfCprGprApfCprApfUprGprAprGprApfCpfUpfCprApfCprAprApfCprAprGpfUpfUpfCpfCpfCpfUpfUpfUprAprGpfUprGprAprGprGprGpfUpfUprAprApfUpfUp3'
116
37278.21 g/mole
1146800 L/(mole·cm)
49.14%
0.87
32.51
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Rhie, A., et al. "Characterization of 2'-fluoro-RNA aptamers that bind preferentially to disease-associated conformations of prion protein and inhibit conversion." Journal of Biological Chemistry, 278 (2003): 39697-705.
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