Aptagen, LLC
DNA
Human Thrombin
Protein
Not given. nM (reported value)
20 mM Tris-HCl (pH 7.4), 1 mM MgCl2, 10 mM NaCl, and 0.05%Tween-20.
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Each U in the sequence is a dU^Az residue. These are azobenzene-modified deoxyuridine.
Azo-1 (ID# 7192)
5’dTpdCpdGpdCpdCpdTpdTpdGpdCpdCpdGpdGpdApdTpdCpdGpdCpdApdGpdApdGprUpdApdApdGpdGpdCpdApdGpdCprUprUpdApdCpdApdApdApdCpdAprUprUpdApdApdGpdGpdCpdApdCpdApdGprUpdGpdGprUpdCpdCpdGprUpdGpdApdGpdCpdCprUpdGpdApdCpdApdCpdCp3’
70
21442.7 g/mole
658400 L/(mole·cm)
55.71%
1.52
32.57
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Morihiro, K.; Hasegawa, O.; Kasahara, Y.; Mori, S.; Kasai, T.; Kuwahara, M.; Obika, S. Azobenzene-modified DNA aptamers evolved by capillary electrophoresis (CE)-SELEX method. Bioorg. Med. Chem. Lett. 2021 Jan 1; 31: 127607. DOI: 10.1016/j.bmcl.2020.127607.
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