Aptamer Details

Phenylalanine-Cp*Rh(III) sensor (ID# 7105)

Aptamer Chemistry: DNA Target: Phenylalanine Antigen/Target Category: Small Organic Affinity (Kd): 3.2 nM (reported value) Binding Conditions/Buffer: 100 μM concentration of monomeric Cp*Rh(III) Binding Temp: 24 °C°C Refolding Protocol: Mix and incubate for 5 min at 95oC, and cool down at room temperature > 30 min Specificity: N/A Comments:

Sequence (): 5'dCpdTpdCpdTpdCpdGpdGpdGpdApdCpdGpdApdCpdGpdGpdApdCpdGpdCpdTpdApdApdTpdCpdTpdTpdApdCpdApdApdGpdGpdGpdCpdGpdTpdApdGpdTpdGpdTpdApdTpdGpdTpdCpdGpdTpdCpdCpdCp3' Length: 51 Molecular Weight: 15707.21 g/mole Extinction Coefficient: 531650 L/(mole·cm) GC Content: 56.86% nmoles/OD₂₆₀: 1.88 μg/OD₂₆₀: 29.54

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yang KA, Barbu M, Halim M, et al. Recognition and sensing of low-epitope targets via ternary complexes with oligonucleotides and synthetic receptors. Nat Chem. 2014;6(11):1003-1008. doi:10.1038/nchem.2058

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Aptamer Details

Phenylalanine-Cp*Rh(III) sensor (ID# 7105)

Yang KA, Barbu M, Halim M, et al. Recognition and sensing of low-epitope targets via ternary complexes with oligonucleotides and synthetic receptors. Nat Chem. 2014;6(11):1003-1008. doi:10.1038/nchem.2058

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