Aptagen, LLC
DNA
SARS-CoV-1 Helicase
Protein
26.8 nM (reported value)
Buffer A (Binding Buffer and Wash Buffer): 50 mM Tris-HCl pH 6.8, 150 mM KCl, 20 mM imidazole, 0.05% Tween-20, 0.1% Triton X-100
Buffer B (Elution Buffer): 30 mL; 50 mM Tris-HCl pH 6.8, 150 mM KCl, 500 mM; imidazole, 0.02% Tween-20, 0.1% Triton
Incubation Time: 30 min
25°C
Heated to 90 C for 5 min and cooled at 48 C in Buffer A
IC50: 17.5 nM
SARS-CoV-1 3'-inverted Thymidine Aptamer NG8 (ID# 7085)
5'dCpdCpdGpdTpdApdApdTpdApdCpdGpdApdCpdTpdCpdApdCpdTpdApdTpdApdGpdGpdGpdGpdApdGpdCpdTpdCpdGpdGpdTpdApdCpdCpdGpdApdApdTpdTpdCpdApdTpdGpdTpdTpdGpdGpdTpdApdGpdTpdTpdGpdGpdCpdTpdTpdGpdTpdGpdTpdTpdCpdGpdTpdGpdTpdGpdTpdTpdApdApdGpdCpdTpdTpdTpdCpdApdGpdApdGpdApdGpdGpdApdTpdCpdCpdTpdTp3'
92
28490.55 g/mole
972910 L/(mole·cm)
46.74%
1.03
29.28
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Shum, K. T., & Tanner, J. A. (2008). Differential Inhibitory Activities and Stabilisation of DNA Aptamers against the SARS Coronavirus Helicase. ChemBioChem, 9(18), 3037-3045. doi:10.1002/cbic.200800491
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