Aptagen, LLC
DNA
Acute myeloid leukemia cells (HL60)
Cells
4.5 nM (reported value)
Binding Buffer (4.5 g/L glucose, 5 mM MgCl2, 0.1 mg/ml tRNA and 1mg/ml BSA, dissolved in Dulbecco's PBS with MgCl2 and CaCl2) containing 20% FBS.
4°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Acute myeloid leukemia cells (KH1C12) (ID# 68)
5'dApdTpdCpdCpdApdGpdApdGpdTpdGpdApdCpdGpdCpdApdGpdCpdApdTpdGpdCpdCpdCpdTpdApdGpdTpdTpdApdCpdTpdApdCpdTpdApdCpdTpdCpdTpdTpdTpdTpdTpdApdGpdCpdApdApdApdCpdTpdGpdGpdApdCpdApdCpdGpdGpdTpdGpdGpdCpdTpdTpdApdGpdTp3'
68
20896.62 g/mole
647700 L/(mole·cm)
47.06%
1.54
32.26
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Sefah, Kwame, et al. "Molecular recognition of acute myeloid leukemia using aptamers." Leukemia, 23 (2009):235-244
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