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Malachite Green (MG-4) (ID# 65)

Aptamer Chemistry: RNA Target: Malchite Green Antigen/Target Category: Small Organic Affinity (Kd): 1 µM (reported value) Binding Conditions/Buffer: Selection buffer: 0.1 M KCl, 5 mM MgCl₂, 10 mM Na-HEPES (pH 7.4) Binding Temp: N/A°C Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Comments:

Sequence (): 5'rGprGprAprUprCprCprCprGprAprCprUprGprGprCprGprAprGprAprGprCprCprAprGprGprUprAprAprCprGprAprAprUprGprGprAprUprCprCp3' Length: 38 Molecular Weight: 12300.52 g/mole Extinction Coefficient: 377500 L/(mole·cm) GC Content: 60.53% nmoles/OD₂₆₀: 2.65 μg/OD₂₆₀: 32.58

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Grate, Dilara and Wilson, Charles. "Laser-mediated, site-specific inactivation of RNA transcripts." PNAS, 96 (1999): 6131-6136

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Aptamer Details

Malachite Green (MG-4) (ID# 65)

Grate, Dilara and Wilson, Charles. "Laser-mediated, site-specific inactivation of RNA transcripts." PNAS, 96 (1999): 6131-6136

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