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Aptamer Details

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Thrombin (15mer) (ID# 64)

Thrombin (15mer)
DNA Thrombin Protein 84 nM (reported value) Selection Buffer: (20 mM Tris-acetate pH 7.4, 40mM NaCl, 5mM KCl, 1mM CaCl2, 1mM MgCl2) 37°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Specific to human thrombin. Does not bind to ovalbumin, human fibrinogen or bovine thrombin. Uehara et al. (2008) found that conjugation of a poly(dA) tail to the 5' end disrupts binding to thrombin.

The 29mer version has higher affinity than the 15mer version, but less inhibitory activity. Aptagen has verified this aptamer in house.
5'dGpdGpdTpdTpdGpdGpdTpdGpdTpdGpdGpdTpdTpdGpdGp3' 15 4726.13 g/mole 143300 L/(mole·cm) 60.00% 6.98 32.98

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Bock, Louis, et al. "Selection of single-stranded DNA molecules that bind and inhibit human thrombin." Nature 355, (1992): 564-566.

S Uehara et al. 30 Poly(dA)-Tailed Thrombin DNA Aptamer to Increase DNase-Resistance and Clotting Inhibitory Activity. Bull. Chem. Soc. Jpn. 81(2008): 1485ƒ??1491

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