Aptagen, LLC
DNA
Unknown Membrane Protien
Protein
10.69 +/- 0.89 nM (reported value)
PBS at 37 C for 45 min with slight agitation
37°C
ssDNA library/pool was heat denatured at 95 C for 10 min and rapidly chilled for 10 min in an ice bath before incubation.
~70% specificity to B. bifidum in comparison to other bacterial species. Including: B. longum, B. animalis, B. breve, B. adolescentis and L. plantarum.
Truncation reduced specificity. Proteinase treatment of target cell-line reduced binding, hinting that target is a protein membrane. ELAA limit of detection 10^4 cfu/mL.
CCFM641-5 (ID# 631)
3'dTpdGpdCpdGpdTpdGpdApdGpdCpdGpdGpdTpdApdGpdGpdCpdCpdCpdCpdTpdApdCpdGpdApdCpdCpdCpdApdCpdTpdGpdTpdGpdGpdTpdTpdGpdGpdGpdCp5'
40
12346 g/mole
367800 L/(mole·cm)
67.50%
2.72
33.57
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Hu, Lujun, et al. "Selection, Characterization and Interaction Studies of a DNA Aptamer for the Detection of Bifidobacterium bifidum" International Journal of Molecular Sciences 10.3390(2017): 833.
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