Aptagen, LLCDNA Tenascin-C (TNC) Protein 150 nM (reported value) Binding Buffer (10 mM Tris-HCl (pH 7.5), 150 mM NaCl, 1 mM MgCl2, 1 mM CaCl2, 0.1% (wt/vol) BSA, 0.1 % (vol/vol) Triton X-100, 0.1% (vol/vol) Tween 20) 4°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Selected using the TNC positive cell line U251. At least a 10-fold decrease in Kd at 22° and 37°.
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