Aptamer Details

S132B-C22 (ID# 565)

Aptamer Chemistry: RNA Target: Botulinum Neuroxin Type A (BoNT/A) Antigen/Target Category: Protein Affinity (Kd): 0.8 nM (reported value) Binding Conditions/Buffer: PBS, pH 7.4, 300mM NaCl, 3mM MgCl2, 5mM DTT, 1mg/ml BSA, 0.2% Tween, 1mg/ml heparin Binding Temp: °C Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Comments:

Sequence (): Length: 127 Molecular Weight: 12930.02 g/mole Extinction Coefficient: 395100 L/(mole·cm) GC Content: 60.00% nmoles/OD₂₆₀: 2.53 μg/OD₂₆₀: 32.73

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Tzuu-Wang Chang, Michael Blank, Pavithra Janardhanan, Bal Ram Singh, Charlene Mello, Michael Blind, Shuowei Cai, "In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxin" Biochemical and Biophysical Research Communications 396 (2010) 854ƒ??860.

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Aptamer Details

S132B-C22 (ID# 565)

Tzuu-Wang Chang, Michael Blank, Pavithra Janardhanan, Bal Ram Singh, Charlene Mello, Michael Blind, Shuowei Cai, "In vitro selection of RNA aptamers that inhibit the activity of type A botulinum neurotoxin" Biochemical and Biophysical Research Communications 396 (2010) 854ƒ??860.

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