Aptagen, LLCRNA Bovine Thrombin Protein 164 nM (reported value) SHMCK+ buffer (20 mM Hepes (pH 7.35), 120 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 0.1% geletin) containing 0.05% Tween 20. Binding Buffer (SHMCK+ buffer containing 0.05% Tween 20) 37°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Ki for inhibiting fibrinogen clot formation was ~493 nM.
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