Aptagen, LLC
RNA
Bovine Thrombin
Protein
164 nM (reported value)
SHMCK+ buffer (20 mM Hepes (pH 7.35), 120 mM NaCl, 5 mM KCl, 1 mM CaCl2, 1 mM MgCl2 and 0.1% geletin) containing 0.05% Tween 20. Binding Buffer (SHMCK+ buffer containing 0.05% Tween 20)
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Ki for inhibiting fibrinogen clot formation was ~493 nM.
Bovine Thrombin (T7 05 RNA) (ID# 55)
5'rGprCprAprAprUprGprGprUprAprCprGprGprUprAprCprUprUprCprCprUprUprUprGprGprAprAprGprAprUprAprGprCprUprGprGprAprGprAprAprCprUprAprAprCprCprAprAprAprAprGprUprGprCprAprCprGprCprUprAprCprUprUprUprGprCprUprAprAp3'
68
21852.22 g/mole
684600 L/(mole·cm)
44.12%
1.46
31.92
X Liu, et al. "RNA aptamers speci?c for bovine thrombin." Journal of Molecular Recognition, 16 (2003): 23-27.
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
X Liu, et al. "RNA aptamers speci?c for bovine thrombin." Journal of Molecular Recognition, 16 (2003): 23-27.
X Liu, et al. "Screening of functional antidotes of RNA aptamers against bovine thrombin." FEBS Lett. 562(2004): 125-128
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