Aptagen, LLC
DNA
Streptavidin
Protein
56.7 nM (reported value)
Binding buffer: 100 mM NaCl, 20 mM, Tris-HCl (pH 7.6), 2 mM MgCl2, 5 mM KCl, 1 mM CaCl2, 0.02% Tween 20
21°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
In binding buffer, heat aptamer to 90°C for 10 minutes, rapidly cool to 4°C for 15 minutes, and allow the solution to incubate at room temperature for 5-8 minutes. This sequence contains the primer regions. The core sequence of the aptamer is: 5'-CTATACTCCACTTTGCTATTTCTCGGTTCCTTCACGCGCCGATCGCAGGCTGATGAATTG-3'.
Streptavidin (31) (ID# 54)
5'dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdCpdTpdApdTpdApdCpdTpdCpdCpdApdCpdTpdTpdTpdGpdCpdTpdApdTpdTpdTpdCpdTpdCpdGpdGpdTpdTpdCpdCpdTpdTpdCpdApdCpdGpdCpdGpdCpdCpdGpdApdTpdCpdGpdCpdApdGpdGpdCpdTpdGpdApdTpdGpdApdApdTpdTpdGpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp3'
96
29413.16 g/mole
903200 L/(mole·cm)
42.71%
1.11
32.57
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Stoltenburg et al. "FluMag-SELEX as an advantageous method for DNA aptamer selection." Analytical and Bioanalytical Chemistry, 383 (2005): 83-91.
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