Aptagen, LLCDNA Thyroid Transcription Factor 1 Protein 3.36 nM (reported value) Ni-NTA magnetic bead (Qiagen) were used with PBS-T (50 mM K2HPO4 pH 7.5, 150 mM NaCl, 0.05% Tween-20) 4°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
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