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Aptamer Details

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14-16 (ID# 501)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA Mouse P68 RNA Helicase (Ddx5) Protein 30.8 nM (reported value) For selection: 5 nmol of aptamer pool in 200 µl of PBS, injected systemically and allowed to circulate for 20 minutes For binding assay: 10 mM Tris-HCl (pH 7.0), 150 mM NaCl, 10% (v/v) glycerol, 2.5 µg/ml tRNA. 10 minute incubation at 37°C For affinity purification: 20 mM HEPES (pH 7.4), 150 mM NaCl, 2 mM CaCl2, 0.1% BSA, 0.1 µM tRNA at 4°C for 2 hours N/A Has better affinity for HA-tagged version of human p68 protein (13.8 nM), but only has an affinity of 190 nM for HA-tagged Tenascin CD. ATPase activity was significantly decreased in the presence of aptamer 14-16. IC50 of aptamer was 0.78 µM compared to that of the library (3.63 µM). An additional ƒ??Uƒ? nucleotide is present at position 55 in supplementary Figure 1-b and in the later-published version of the paper available at http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2795795/. This sequence was used to predict the secondary structure.
5'rGprGprGp rAprGprGp rApfCprGp rApfUprGp fCprGprGp fCprAprGp fUprGpfCp fCpfCprAp rApfCpfCp rGprGprAp rApfCprAp rApfCprAp rApfCpfCp rApfCpfCp rGprGpfCp rGprGpfCp fUpfCpfCp fUprGpfCp fUpfCprAp rGprApfCp rGprApfCp fUpfCprGp fCpfUprGp rAprGprGp rApfUpfCp fCprGprAp rGprAp3' 88 25949.66 g/mole 769500 L/(mole·cm) 65.00% 1.30 33.72

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Mi et al. ƒ??In vivo selection of tumor-targeting RNA motifsƒ?. Nature Chemical Biology 1(2010): 22-24. DOI: 10.1038/NCHEMBIO.277

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