Aptagen, LLC
DNA
IgE
Protein
23 nM (reported value)
Binding buffer: PBS and 1 mM MgCl2 (pH 8.0)
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Selectively bind human IgE over human IgG and mouse IgE.
The selectivity was not selected for, but rather a byproduct of the high affinity for human IgE.
IgE (4.4.12) (ID# 50)
5'dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdTpdGpdApdApdApdCpdApdTpdApdGpdCpdApdTpdApdTpdTpdTpdApdCpdTpdTpdApdTpdGpdTpdCpdGpdCpdCpdTpdTpdGpdCpdCpdGpdGpdTpdTpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp3'
78
24050.67 g/mole
753800 L/(mole·cm)
47.44%
1.33
31.91
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Mendonsa, Shaun D. and Bowser, Michael T. "In Vitro Selection of High-Affinity DNA Ligands for Human IgE Using Capillary Electrophoresis." Analytical Chemistry, 76 (2004): 5387-5392.
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