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Aptamer Details

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IgE (4.4.12) (ID# 50)

IgE (4.4.12)
DNA IgE Protein 23 nM (reported value) Binding buffer: PBS and 1 mM MgCl2 (pH 8.0) 25°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding program to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Selectively bind human IgE over human IgG and mouse IgE. The selectivity was not selected for, but rather a byproduct of the high affinity for human IgE.
5'dApdGpdCpdApdGpdCpdApdCpdApdGpdApdGpdGpdTpdCpdApdGpdApdTpdGpdTpdGpdApdApdApdCpdApdTpdApdGpdCpdApdTpdApdTpdTpdTpdApdCpdTpdTpdApdTpdGpdTpdCpdGpdCpdCpdTpdTpdGpdCpdCpdGpdGpdTpdTpdCpdCpdTpdApdTpdGpdCpdGpdTpdGpdCpdTpdApdCpdCpdGpdTpdGpdApdAp3' 78 24050.67 g/mole 753800 L/(mole·cm) 47.44% 1.33 31.91

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Mendonsa, Shaun D. and Bowser, Michael T. "In Vitro Selection of High-Affinity DNA Ligands for Human IgE Using Capillary Electrophoresis." Analytical Chemistry, 76 (2004): 5387-5392.

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