Aptagen, LLC
DNA
Kanamycin A
Small Organic
5.1 nM (reported value)
Selection Buffer: 100 mM NaCL, 20 mM Tris-HCl pH 7.6, 2 mM MgCl2, 5 mM KCl, and 1 mM CaCl2
21°C
90°C for 8 min, immediately cooled, 4 °C for 10 min.
Lowercase letters denote constant regions. This aptamer was selected using capture-SELEX in which the DNA was immoblized using DNA complimentary to the middle constant region and was eluted by the target during the selection process.
Kanamycin A (#13_82) (ID# 498)
5'dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdCpdApdGpdGpdGpdCpdGpdGpdTpdApdTpdGpdApdGpdGpdCpdTpdCpdGpdApdTpdCpdApdApdGpdGpdTpdCpdGpdGpdApdGpdCpdGpdApdGpdApdApdTpdTpdTpdTpdTpdTpdCpdGpdCpdGpdGpdApdGpdTpdCpdGpdGpdCpdTpdGpdGpdApdTpdCpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp3'
98
30429.82 g/mole
961600 L/(mole·cm)
47.96%
1.04
31.64
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Stoltenburg R, Nikolaus N, Strehlitz B.(2012) Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics. J Anal Methods Chem. 2012:doi:10.1155/2012/415697
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