Aptagen, LLC
DNA
Recombinant H3 cluster IV
Protein
7.1 nM (reported value)
For Selection: 50 mM NaH2PO4, 50 mM NaCl, and 20 mM imidazole (pH 8.0) for 1 hour on a rotisserie shaker For Binding Affinity: 1% fish gelatin, gentle shaking for 1 hour
25°C
95°C for 10 minutes followed by a 10 minute incubation at room temperature
Shown to bind specifically to the H3 subtype compared to recombinant HA proteins from other subtypes.
Binding region located at residues 8-40. At 2.5 uM, completely inhibited agglutination of turkey red blood cells in presence of 16 HA units/50 ul.
Swine Influenza A Virus - H3N2 (HA68) (ID# 494)
5'dApdCpdTpdCpdCpdGpdTpdCpdApdTpdCpdTpdTpdTpdApdGpdTpdGpdGpdCpdCpdCpdCpdApdApdTpdGpdTpdCpdGpdTpdTpdApdTpdCpdApdCpdCpdGpdAp3'
40
12157.93 g/mole
371300 L/(mole·cm)
50.00%
2.69
32.74
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Wongphatcharachai, Manoosak, et al. "Neutralizing DNA Aptamers against Swine Influenza H3N2 Viruses." Journal of Clinical Microbiology 2013, 51(1):46.
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