Aptagen, LLCRNA Sialyl Lewis X Other 0.085 nM (reported value) Binding Buffer (150 mM NaCl, 20 mM Hepes (pH 7.4), 1 mM CaCl2, 1 mM MgCl2) RT°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. The reported sequence contains Y(pyrimidine) at site 46 and 67. We've chosen uracil (U) to replace the arbitrary Y. The reported sequence contains R(purine) at site 101. We've chosen adenine(A) to replace the arbitrary R.
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