Aptagen, LLC
![Peroxidase DNAzyme ([B7]-3-0)](/wp-content/uploads/g-quartet.png "Peroxidase DNAzyme ([B7]-3-0)")
DNA
Hemin
Protein
29 nM (reported value)
Binding buffer: 20 mM Tris-AcOH (pH 8.0), 100 mM NaCl, 200 mM KCl, 5 mM MgCl2, 0.5% Triton X-100, and 5% DMSO.
Peroxidase activity was measured using the ABTS-H2O2 system. RT°C Heated at 95°C for 5 min in 40KT buffer, cooled on ice for 5 min, and placed at RT.
40KT buffer: 50 mM MES (pH 6.5), 100 mM Tris acetate, 40 mM potassium acetate, 1% DMSO, and 0.05% Triton X Forms a G-quadruplex structure that enhances the natural peroxidase activity of hemin
Peroxidase DNAzyme ([B7]-3-0) (ID# 469)
Peroxidase activity was measured using the ABTS-H2O2 system. RT°C Heated at 95°C for 5 min in 40KT buffer, cooled on ice for 5 min, and placed at RT.
40KT buffer: 50 mM MES (pH 6.5), 100 mM Tris acetate, 40 mM potassium acetate, 1% DMSO, and 0.05% Triton X Forms a G-quadruplex structure that enhances the natural peroxidase activity of hemin
5'dApdTpdTpdGpdGpdGpdApdGpdGpdGpdApdTpdTpdGpdGpdGpdTpdGpdGpdGp3'
20
6349.19 g/mole
203600 L/(mole·cm)
60.00%
4.91
31.18
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Zhu L, et al. (2012) In vitro selection of highly efficient G-quadruplex-based DNAzymes. Anal. Chem. 84: 8383-8390.
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