Aptamer Details

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MCP-1 (ADR7) (ID# 46)

* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.

Aptamer Chemistry: 2'-F-RNA Target: Monocyte chemoattractant protein-1 (mouse) Antigen/Target Category: Protein Affinity (Kd): 180 pM (reported value) Binding Conditions/Buffer: Selection Buffer (110 mM NaCl, 1 mM MgCl2, 20 mM HEPES (pH 7.0), 1 mM CaCl2, 5 mM KCl, 0.001% HSA, 0.05% Tween 20) Binding Temp: 37°C Refolding Protocol: None given. Comments:

Sequence (): 5'rGprGprGprAprGprGprApfCprGprApfUprGpfCprGprGprGprAprApfCpfUpfCprApfCpfCprGprGprGprAprAprGprAprAprGpfCpfCpfCprGpfUpfUpfCpfCprGpfUpfCprApfCprAprGprApfCprApfUprGpfUpfUpfCpfCprGpfCprApfUpfCprGpfUpfCpfCpfUpfCpfCpfCp3' Length: 70 Molecular Weight: 22580.5 g/mole Extinction Coefficient: 670700 L/(mole·cm) GC Content: 62.86% nmoles/OD₂₆₀: 1.49 μg/OD₂₆₀: 33.67

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Rhodes, Andrew, et al. "The generation and characterisation of antagonist RNA aptamers to MCP-1." FEBS Letter, 506 (2001): 85-90.

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Aptamer Details

MCP-1 (ADR7) (ID# 46)

Rhodes, Andrew, et al. "The generation and characterisation of antagonist RNA aptamers to MCP-1." FEBS Letter, 506 (2001): 85-90.

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