Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
Peptide
Anti-human platelet antigen antibodies (HPA-1a)
Protein
N/A nM (reported value)
Buffer for ELISA assay: PBS.
20-25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Aptamer consists of a selected dodecapeptide (VVAGDDPREDTW) inserted into the thioredoxin protein with a C-terminal poly-histone tail. The dodecapeptide mimics human platelet antigen-1 and binds to many of the antibodies that target HPA-1a, though not all of them.
Anti-human platelet antigen antibodies (Trx-HPA-1a) (ID# 421)
5'MetMetSerAspLys IleIleHisLeuThr AspAspSerPheAsp ThrAspValLeuLys AlaAspGlyAlaIle LeuValAspPheTrp AlaGluTrpCysGly ProValValAlaGly AspAspProArgGlu AspThrTrpGlyPro CysLysMetIleAla ProIleLeuAspGlu IleAlaAspGluTyr GlnGlyLysLeuThr ValAlaLysLeuAsn IleAspGlnAsnPro GlyThrAlaProLys TyrGlyIleArgGly IleProThrLeuLeu LeuPheLysAsnGly GluValAlaAlaThr LysValGlyAlaLeu SerLysGlyGlnLeu LysGluPheLeuAsp AlaAsnLeuValAsp LeuGlnHisHisHis HisHisHis3'
141
16956.67 g/mole
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
J Thibaut et al. A novel assay for the detection of anti-human platelet antigen antibodies (HPA-1a) based on peptide aptamer technology. haematologica 97(2012): 696-704.
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