Aptagen, LLC
RNA
Yeast phenylalanine tRNA
Small Organic
12 nM (reported value)
Selection Buffer (250 mM NaCl, 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 2 mM spermidine, 0.2 mM EDTA)
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Yeast phenylalanine tRNA (B2) (ID# 42)
5'rGprGprGprAprAprUprUprCprCprGprCprGprUprGprUprGprCprUprAprCprGprUprAprUprCprUprUprCprAprGprGprCprGprGprUprAprAprCprUprAprAprCprUprGprUprGprCprUprGprAprGprUprCprUprAprAprUprCprUprUprUprGprUprGprAprGprGprGprAprCprGprGprUprAprAprCprAprUprAprUprGprGprUprUprCprCprCprGprCprGprUprGprGprUprCprCprGprUprUprCprGprGprGprAprUprCprCprUprCp3'
109
35011.9 g/mole
1060400 L/(mole·cm)
53.21%
0.94
33.02
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Scarabino, Daniela, et al. "tRNA prefers to kiss." EMBO Journal 18 (1999): 4571-4578
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