Aptamer Details

HIV-1 Rev Peptide (L1-Rev) (ID# 413)

Aptamer Chemistry: RNA Target: HIV-1 Rev Peptide Antigen/Target Category: Peptide Affinity (Kd): 18 µM (reported value) Binding Conditions/Buffer: Reaction buffer: 20 mM Tris-HCl, pH 7.5, 50 mM NaCl, and 60 mM MgCl₂ Binding Temp: 25°C Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Comments:

Sequence (): 5'rGprGprAprCprCprUprCprGprGprCprGprAprAprAprGprCprCprGprGprUprAprAprCprGprCprCprAprCprAprAprGprUprCprGprGprAprGprGprGprUprAprAprGprAprUprCprUprGprAprCprAprGprGprAprAprCprUprGprGprGprUprGprAprCprGprGprAprGprGprUprUprAprGprGprUprGprCp3' Length: 77 Molecular Weight: 25129.29 g/mole Extinction Coefficient: 774600 L/(mole·cm) GC Content: 59.74% nmoles/OD₂₆₀: 1.29 μg/OD₂₆₀: 32.44

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Hesslberth et al. "Simultaneous Detection of Diverse Analytes with an Aptazyme Ligase Array." Analytical Biochemistry, 312, (2003):106-112.

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Aptamer Details

HIV-1 Rev Peptide (L1-Rev) (ID# 413)

Hesslberth et al. "Simultaneous Detection of Diverse Analytes with an Aptazyme Ligase Array." Analytical Biochemistry, 312, (2003):106-112.

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