Aptagen, LLC
* The secondary structure may not be accurate because of MFOLD limitations on chemistries other than RNA/DNA.
2'-F-RNA
CD4 protein found on CD4+ T-Cells
Protein
0.5 nM (reported value)
Binding Buffer: PBS containing 1 mM MgCl2
RT°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
This aptamer was also used for cell capture. (Q Zhou et al. "Aptamer-containing surfaces for selective capture of CD4 expressing cells." Langmuir 28(2012):12544-12549.)
CD4 (aptamer 12) (ID# 369)
5' rGprGprGprAprGprApfCprAprAprGp rAprApfUprAprAprApfCprGpfCpfUp fCprAprAprGpfUprGprApfCprGpfUp fCpfCpfUprGprApfUpfCprGprApfUp fUprGpfUprGpfCprApfUpfUpfCprGp rGpfUprGpfUprGprApfCprGprApfUp fCpfUpfUpfUpfCprGprApfCprAprGp rGprAprGprGpfCpfUpfCprApfCprAp rApfCprAprGprGpfCp3'
89
27852.63 g/mole
861400 L/(mole·cm)
51.16%
1.16
32.33
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
K A Davis et al. "Staining of cell surface human CD4 with 2'-F-pyrimidine-containing RNA aptamers for flow cytometry." Nucleic Acids Res 26(1998):3915-3924.
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