Aptagen, LLC
DNA
H4 N-terminal tail
Protein
1.3 nM (reported value)
Low-salt conditions: 100 mM NaCl, 5 mM MgCl2, and 10 mM HEPES (pH 7.5).
High-salt conditions: 500 mM NaCl, 10 mM MgCl2, and 10 mM HEPES (pH 7.5). N/A°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Kd values for other histones (low-/high-salt concentrations): H3 (14/273 nM), H2 A (204/4050 nM), and H2 B (84/825 nM). Affinities for H3, H2 A, and H2 B histones are also provided.
Histone H4 N-terminal tail (4.33) (ID# 355)
High-salt conditions: 500 mM NaCl, 10 mM MgCl2, and 10 mM HEPES (pH 7.5). N/A°C NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Kd values for other histones (low-/high-salt concentrations): H3 (14/273 nM), H2 A (204/4050 nM), and H2 B (84/825 nM). Affinities for H3, H2 A, and H2 B histones are also provided.
5'dTpdGpdGpdTpdGpdGpdGpdGpdTpdTpdCpdCpdCpdGpdGpdGpdApdGpdGpdGpdCpdGpdGpdCpdTpdApdCpdGpdGpdGpdTpdTpdCpdCpdGpdTpdApdApdTpdCpdApdGpdApdTpdTpdTpdGpdTpdGpdTp3'
50
15592.13 g/mole
473300 L/(mole·cm)
60.00%
2.11
32.94
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Yu et al. "Aptamers can Discriminate Alkaline Proteins with High Specificity." Chem. Biochem. , 12(2011): 2659-2666.
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