Aptagen, LLC
DNA
Mercury II (Hg2+)
Other
40 nM (reported value)
3-(N-morpholino)propanesulfonic acid (10mM, pH 7.0, NaCl (25mM), NaNO3 (500mM), and ethylenediamine (0.1 mM)
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Authors attached dabcyl (quencher) and 6-fluorescein (fluorophore) to the 5' and 3' ends of the aptamer. In the presence of Hg2+ ions, the aptamer forms a hairpin with T-Hg2+-T bonds. This brings the quencher into proximity of the fluorophore.
Mercury II (Hg2+) (ID# 325)
5'dTpdTpdCpdTpdTpdTpdCpdTpdTpdCpdCpdCpdCpdTpdTpdGpdTpdTpdTpdGpdTpdTp3'
22
6590.34 g/mole
178600 L/(mole·cm)
36.36%
5.60
36.90
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
A. Ono and H. Togashi. "Highly selective oligonucleotide-based sensor for mercury(II) in aqueous solutions." Angew. Chem. Int. Ed. 43(2004):4300-4302.
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