Aptagen, LLC
DNA
Thrombin
Protein
0.5 nM (reported value)
50 mM Tris-HCl (pH 7.5), 100 mM NaCl, 1 mM MgCl2
37°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Image does not show G quartet formation. The 29mer version has higher affinity than the 15mer version, but less inhibitory activity.
Thrombin (29mer) (ID# 315)
5'dApdGpdTpdCpdCpdGpdTpdGpdGpdTpdApdGpdGpdGpdCpdApdGpdGpdTpdTpdGpdGpdGpdGpdTpdGpdApdCpdTp3'
29
9085.94 g/mole
283600 L/(mole·cm)
62.07%
3.53
32.04
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Tasset, D. (1997). Oligonucleotide inhibitors of human thrombin that bind distinct epitopes. J. Mol. Biol., 1997(272), 688-698.
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