Aptagen, LLC
DNA
Bovine Catalase
Protein
237 nM (reported value)
CE Selection Buffer (1X TGK): 25 mM Tris, 192 mM glycine, 5 mM potassium phosphate buffer (pH 8.3).
CE Run Buffer: 3X TGK N/A°C Heated to 94°C for 10 min and then cooled at 0.5° C/s to 20°C. At least a 100-fold decrease in affinity to lysozyme, trypsinogen, chymotrypsinogenA, and myoglobin relative to the target. The Kd value listed was determined using affinity capillary electrophoresis. The Kd value using a fluorescence intensity assay was 430 nM.
Bovine Catalase (CAT 1) (ID# 307)
CE Run Buffer: 3X TGK N/A°C Heated to 94°C for 10 min and then cooled at 0.5° C/s to 20°C. At least a 100-fold decrease in affinity to lysozyme, trypsinogen, chymotrypsinogenA, and myoglobin relative to the target. The Kd value listed was determined using affinity capillary electrophoresis. The Kd value using a fluorescence intensity assay was 430 nM.
5'dCpdTpdTp dCpdTpdGp dCpdCpdCp dGpdCpdCp dTpdCpdCp dTpdTpdCp dCp dGpdApdCpdCpdTpdApdGp dCpdApdGpdTpdGpdGpdApdCpdAp dTpdGpdTpdGpdGpdCpdApdGpdGpdGpdTpdGp dApdApdGpdTpdGpdGpdCpdAp dTpdCpdGpdTpdCp dGpdGpdApdGpdApdCp dGpdApdGp dApdTpdApdGpdGpdCp dGpdGpdAp dCpdApdCp dTp3'
87
25356.41 g/mole
774800 L/(mole·cm)
60.98%
1.29
32.73
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Ashley, J. et al. Selection of bovine catalase aptamers using non-selex. Electrophoresis 33(2012):2783-2789.
Have your aptamer oligo synthesized ORDER NOW
We are always looking for ways to improve. Please tell us what you think.