Aptamer Details

TLR3-ECD (Family-1) (ID# 211)

Aptamer Chemistry: RNA Target: Toll-like receptor 3 Ectodomain Antigen/Target Category: Protein Affinity (Kd): 2.1 nM (reported value) Binding Conditions/Buffer: Binding buffer: 2 mM HEPES-NaOH (pH 7.6), 3 mM MgCl₂, and 100 mM NaCl Binding Temp: N/A°C Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Comments:

Sequence (): 5'rGprGprUprAprGprAprUprAprCprGprAprUprGprGprAprUprAprCprCprCprCprCprUprGprUprGprGprCprCprCprGprUprCprAprAprCprAprCprAprGprGprGprGprAprAprGprUprGprGprCprAprUprGprAprCprGprCprGprCprAprGprCprCprAp3' Length: 64 Molecular Weight: 20703.58 g/mole Extinction Coefficient: 628900 L/(mole·cm) GC Content: 60.94% nmoles/OD₂₆₀: 1.59 μg/OD₂₆₀: 32.92

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Watanabe et al. "Isolation of RNA aptamers against human Toll-like receptor 3 ectodomain." Nucleic Acids Symposium Series No. 50 (2006): 251-252.

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Aptamer Details

TLR3-ECD (Family-1) (ID# 211)

Watanabe et al. "Isolation of RNA aptamers against human Toll-like receptor 3 ectodomain." Nucleic Acids Symposium Series No. 50 (2006): 251-252.

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