Aptamer Details

YPEN-1 Endothelial Cells (III.1) (ID# 210)

Aptamer Chemistry: DNA Target: YPEN-1 Endothelial Cells Antigen/Target Category: Cells Affinity (Kd): N/A nM (reported value) Binding Conditions/Buffer: Selection buffer: 50 mM Tris-HCl (pH 7.4), 5 mM KCl, 100 mM NaCl, 1 mM MgCl₂, and 0.1% NaN3 with 1 ug/uL yeast tRNA. Binding Temp: RT°C Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding. Comments:

Sequence (): 5'dApdTpdApdCpdCpdApdGpdCpdTpdTpdApdTpdTpdCpdApdApdTpdTpdApdGpdGpdCpdGpdGpdTpdGpdCpdApdTpdTpdGpdTpdGpdGpdTpdTpdGpdGpdTpdApdGpdTpdApdTpdApdCpdApdTpdGpdApdGpdGpdTpdTpdTpdGpdGpdTpdTpdGpdApdGpdApdCpdTpdApdGpdTpdCpdGpdCpdApdApdGpdApdTpdApdTpdApdGpdApdTpdApdGpdTpdApdApdGpdTpdGpdCpdApdApdTpdCpdTp3' Length: 96 Molecular Weight: 29854.53 g/mole Extinction Coefficient: 961400 L/(mole·cm) GC Content: 39.58% nmoles/OD₂₆₀: 1.04 μg/OD₂₆₀: 31.05

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Blank et al. "Systematic Evolution of a DNA Aptamer Binding to Rat Brain Tumor Microvessels." The Journal of Biological Chemistry, >b>276(2001): 16464-16468.

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Aptamer Details

YPEN-1 Endothelial Cells (III.1) (ID# 210)

Blank et al. "Systematic Evolution of a DNA Aptamer Binding to Rat Brain Tumor Microvessels." The Journal of Biological Chemistry, >b>276(2001): 16464-16468.

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