Aptagen, LLC
RNA
HIV-1 Integrase
Protein
10 nM (reported value)
Buffer A (50 mM HEPES, 1 mM DTT, 1 mM APMSF) Binding Buffer (50 mM Na-HEPES (pH 7.5), 250 mM NaCl, 2 mM DTT, 10 mM MnCl2, 5 mM CHAPS)
N/A°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
HIV-1 Integrase (P5) (ID# 129)
5'rGprGprGprAprGprCprUprCprAprGprAprAprUprAprAprAprCprGprCprUprCprAprAprCprCprAprGprUprCprUprUprGprUprGprGprCprUprUprUprGprAprAprAprGprAprGprAprGprGprAprGprUprGprUprUprCprGprAprCprAprUprGprAprGprGprCprCprCprGprGprAprUprCprCprGprGprCp3'
77
24933.1 g/mole
767300 L/(mole·cm)
54.55%
1.30
32.49
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Allen, P., et al. "Isolation of High-Affinity RNA Ligands to HIV-1 Integrase from a Random Pool." Virology, 209(1995): 327-336.
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