Aptamer Details

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LepDapt-5561 (ID# 9713)

Aptamer Chemistry: DNA

Target: Lipl32 protein

Antigen/Target Category: Protein

Affinity (Kd): 48.36 (+/-) 8.03 from direct ELASA nM (reported value)

Binding Conditions/Buffer: Obtained in cycle 9. Tripartite partitioning mechanism used to develop the aptamer. Washing - 1X PBST Binding takes place in presence of blocking agents to prevent non-specific binding.

Binding Temp: 25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Specificity: Specific to LipL32 protein. Has been tested against a non-pathogenic Leptospira variant and a dengue virus serotype 2 NS1 protein. Used in sandwich ELISA as a biotinylated detection antibody to detect LipL32

Sequence ():

Length: 80

Molecular Weight: 25004.2 g/mole

Extinction Coefficient: 794700 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Yeoh, T.S., Cheah, HL., Yoke, L.F. et al. Isolation of DNA aptamers by Tripartite-hybrid-high throughput SELEX (Tripartite-hybrid-HT SELEX) for the detection of pathogenic Leptospira. World J Microbiol Biotechnol 41, 454 (2025). https://doi.org/10.1007/s11274-025-04637-8

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