Aptagen, LLC
Brb7 (ID# 9633)
DNA
Bilirubin
Small Organic
203 nM (reported value)
Selection buffer - 200 mM NaCl, 10 mM phosphate, 2 mM MgCl2, pH 7.6. The randomized aptamer library was annealed with the biotinylated capture strand in selection buffer. Streptavidin-agarose resin was loaded, and biotin-ssDNA complex was added into it. Bilirubin was loaded onto the library and the eluted ssDNA was obtained through gravity flow.
25°C
NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.
Specifically binds to Bilirubin in a dose-dependent manner. It does not bind with high affinity to Biliverdin, or other structurally similar biomolecules.
dApdTpdApdApdGpdCpdTpdCpdTpdTpdApdGpdCpdGpdCpdGpdTpdGpdTpdTpdTpdApdCpdCpdApdCpdCpdTpdTpdTp
30
9123 g/mole
276100 L/(mole·cm)
Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.
Yachen Xie, Yunus A. Kaiyum, Lide Gu, Yibo Liu, Philip E. Johnson, and Juewen Liu ACS Chemical Biology 2025 20 (8), 2028-2037 DOI: 10.1021/acschembio.5c00438
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