Aptamer Details

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mApoc46 (ID# 9541)

Aptamer Chemistry: DNA

Target: Patient-derived high-grade serous ovarian cancer (HG-SOC) cells

Antigen/Target Category: Cells

Affinity (Kd): 150 ± 50 nM for pSOC cells nM (reported value)

Binding Conditions/Buffer: Incubate 100–150 nM FAM-aptamer with 1×10⁵ target cells in DPBS (Ca²⁺/Mg²⁺, pH 7.4) + 0.05 g/L BSA + 2 µg/mL salmon sperm DNA at 4 °C for 20–30 min, then wash with DPBS.

Binding Temp: 4 °C for cell binding; 25 °C for SPR; various temps for stability tests at 37 °C

Refolding Protocol: Heat the aptamer in binding buffer at 95 °C for 5 min, snap-cool on ice for 5 min, then equilibrate at 4 °C before use.

Sequence ():

Length: 59 nucleotides

Molecular Weight: ≈ 18–19 kDa

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wang, J., Fang, X., Zhang, C., Ji, H., Pang, Q., Li, X., Luo, Z., Wu, Q., & Zhang, L. (2021). Development of aptamer-based molecular tools for rapid intraoperative diagnosis and in vivo imaging of serous ovarian cancer. ACS Applied Materials & Interfaces, 13(19), 22048–22061. https://doi.org/10.1021/acsami.1c02072

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