Aptamer Details

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Apt 21-25 (ID# 9498)

Aptamer Chemistry: DNA

Target: Equine Herpesvirus Type 1 (EHV-1)

Antigen/Target Category: Small Organic

Binding Conditions/Buffer: G-quadruplex–forming oligonucleotides were prepared at a final concentration of 7.5 μM in 25 mM Tris–HCl buffer (pH 7.0) containing varying NaCl concentrations. The mixtures were statically incubated to promote proper folding. Crystal violet (CV) was then added to a final concentration of 15 μM, and the mixtures were incubated at 30 °C for 1 h.

Binding Temp: 30°C

Refolding Protocol: 10-min heat treatment at 95 °C

Sequence ():

Length: 30

Molecular Weight: 9498.2 g/mole

Extinction Coefficient: 306200 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Nahid Davoudi, Mandana Behbahani, Hassan Mohabatkar, Ghasem Dini, Mehran Bakhshesh. A novel G-quadruplex aptamer with high affinity for the specific detection of equine herpesvirus type 1: Comprehensive biophysical and analytical characterization, Talanta, Volume 297, Part B. 2026. 128713. ISSN 0039-9140. https://doi.org/10.1016/j.talanta.2025.128713.

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