Aptamer Details

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NC42 (ID# 9450)

Aptamer Chemistry: DNA

Target: Cocaine

Antigen/Target Category: Small Organic

Affinity (Kd): 282 nM (reported value)

Binding Temp: 75.2°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 46

Molecular Weight: 14290.3 g/mole

Extinction Coefficient: 457000 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Obtin Alkhamis, Caleb Byrd, Juan Canoura, Adara Bacon, Ransom Hill, Yi Xiao, Exploring the relationship between aptamer binding thermodynamics, affinity, and specificity, Nucleic Acids Research, Volume 53, Issue 6, 11 April 2025, gkaf219, https://doi.org/10.1093/nar/gkaf219

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