Aptamer Details

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Aptamer CIV6 against Acyclic Guanosine Analogues (ID# 9411)

Aptamer Chemistry: DNA

Target: Acyclic Guanosine Analogues: acyclovir (ACV), famciclovir (FCV), ganciclovir (GCV), penciclovir (PCV), and valaciclovir (VACV)

Antigen/Target Category: Small Organic

Affinity (Kd): 32.67±4.127 (ACV), 47.91±13.47 (GCV), 33.29±5.851 (PCV), 47.35±10.42 (FCV), 44.26±6.744 (VACV) nM (reported value)

Binding Conditions/Buffer: 1mL BB, (50 mMTris-HCl, 5 mM KCl, 100 mM NaCl, 1 mM MgCl2, pH 7.4)

Binding Temp: 37 °C °C

Refolding Protocol: heated at 95 °C for 10 min, cooled down, and incubated at 37°C for 2 h.

Specificity: Via a fluorescence method of 100 nM FAM labeled aptamer candidate incubated at 37 °C, 130 rpm in the dark for 20 min with Graphene Oxide. Afterward, positive target or negative target was added to the above solute at 37 °C in dark conditions for 1 hour.

Comments: The different dissociation constants (Kd) are associated with the five distinct acyclic guanosine analogues described in the Target Section.

Sequence ():

Length: 80

Molecular Weight: 24722 g/mole

Extinction Coefficient: 778400 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Ren, L., Qi, S., Khan, I. M., Wu, S., Duan, N., & Wang, Z. (2021). Screening and application of a broad-spectrum aptamer for acyclic guanosine analogues. Analytical and bioanalytical chemistry, 413(19), 4855–4863. https://doi.org/10.1007/s00216-021-03446-w

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