Aptamer Details

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Aptamer Apt-4 against TIMP-1 Protein (ID# 9399)

Aptamer Chemistry: DNA

Target: Tissue inhibitor of metalloproteinases 1

Antigen/Target Category: Protein

Affinity (Kd): 0.41 nM (reported value)

Binding Conditions/Buffer: Dulbecco’s phosphate buffered saline solution (DPBS). It contains 138 mM NaCl, 2.67 mM KCl, 8.1 mM Na2HPO4, 1.47 mM KH2PO4, 1 mM CaCl2, and 0.5 mM MgCl2

Binding Temp: 25 °C °C

Refolding Protocol: Was Not Provided

Specificity: Apt-4 has high specificity for TIMP-1 and minimal binding to proteins: AFP, CA199, CEA, BSA, 8His peptide

Comments: Used a magnetic beads-based SELEX process combined with qPCR to select aptamers over seven rounds.

Sequence ():

Length: 56

Molecular Weight: 17187.1 g/mole

Extinction Coefficient: 519700 L/(mole·cm)

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Wang, Q., Li, Y., Yao, L., Li, H., Zhang, L., Wang, Y., Li, J., Chen, T., Chai, K., Gao, J., Gao, J., Su, L., & Li, X. (2025). High-affinity ssDNA aptamer and chemiluminescent aptasensor for TIMP-1 detection in human serum. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 41(2), 119–126. https://doi.org/10.1007/s44211-024-00673-w

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