Aptamer Details

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hVap1_Stem (ID# 7922)

Aptamer Chemistry: DNA

Target: VEGF

Antigen/Target Category: Protein

Affinity (Kd): 5.6 nM (reported value)

Binding Conditions/Buffer: APBB; 10 mM HEPES, pH 7.9, 125 mM NaCl, 25 mM KCl, and 1 mM MgCl2

Binding Temp: 23-25°C

Refolding Protocol: NA If the oligo is a known aptamer sequence: For binding studies, perform a refolding protocol to ensure proper function (i.e. binding to antigen or target). Refer to the aptamer reference source for the appropriate refolding parameters and binding conditions. Note: it is unknown whether aptamer functions properly without refolding.

Sequence ():

Length: 48

Molecular Weight: 14775.61

Extinction Coefficient: 453400

Note: Information on this aptamer oligo was obtained from the literature and hasn't been validated by Aptagen.

Reference:

Meng, Hsein-Wei, and John Pagano. "Discovering Aptamers by Cell-SELEX against Human Soluble Growth Factors Ectopically Expressed on Yeast Cell Surface." Plos-One 9.3 (2014): 1-12. Print.

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